SeqTUI is a fast terminal-based viewer and command-line toolkit for sequences. View, translate, convert (to FASTA), and combine sequences aligned or not — all from the terminal.
This is free and open source software.
Features include:
- Multi-Format Support: FASTA, PHYLIP, and NEXUS formats with auto-detection.
- Color-Coded: Nucleotides and amino acids displayed with distinct background colors.
- NT → AA Translation: 33 NCBI genetic codes, handling ambiguity codes (R, Y, N).
- Concatenation & Supermatrix: Combine multiple alignments, fill missing with gaps.
- SNP Extraction: Export isolated biallelic SNPs to VCF with flanking distance filter.
- Sticky Names: Sequence identifiers remain visible while scrolling.
- Vim-style Navigation: h/j/k/l, w/b/e, Ctrl+U/D, search with / and ?.
- Command-Line Mode: Batch convert, translate, combine — pipe-friendly output.
- Large File Support: Tested on alignments larger than 500 MB.
Website: github.com/ranwez-search/SeqTUI
Support:
Developer: Vincent Ranwez
License: MIT License

SeqTUI is written in Rust. Learn Rust with our recommended free books and free tutorials.
| Explore Linux | |
|---|---|
| Best Free and Open Source Software - A huge collection of recommended free and open source software covering every major category. | |
| In-depth Linux Software Reviews - Detailed assessments of Linux software covering features, usability and overall quality. | |
| The Big List of Active Linux Distros - An extensive collection of actively developed Linux distributions. | |
| Open Source Alternatives - Alternatives to software and services from Google, Microsoft, Apple, Adobe, IBM, Autodesk, Oracle and others. | |
| Essential Linux System Tools - Indispensable utilities for monitoring, maintaining and managing Linux systems. | |
| Linux Productivity Tools - Useful utilities for improving productivity and streamlining everyday tasks. | |
| Alternatives to Popular CLI Tools - Modern alternatives to familiar command-line utilities supplied with Linux systems. | |
| Awesome Free Linux Game Tools - Tools, utilities and companion software that improve gaming on Linux. | |
| Machine Learning on Linux - Machine learning and deep learning software, self-hosted applications and practical tools. | |
| Saving Money with Linux - Practical ways to reduce electricity consumption and lower computing costs with Linux. | |
| Linux Candy - Fun, entertaining and sometimes delightfully quirky software from the lighter side of Linux. | |

Please read our Comment Policy before commenting.