FastQC is a quality control tool for high throughput sequence data.
Written in Java, it is designed to spot potential problems in sequencing datasets by running a set of analyses on raw sequence files and producing a report which summarises the results. It can be used as an interactive graphical application or run in a non-interactive mode as part of a pipeline.
This is free and open source software.
Key Features
- Runs quality control analyses on high throughput sequencing data.
- Works with raw sequence files in FASTQ or BAM format.
- Produces reports summarising analysis results.
- Highlights areas where a sequencing library may appear unusual.
- Can be used as an interactive graphical application.
- Can be run in a non-interactive mode as part of a pipeline.
- Cross-platform support – runs under Linux, macOS, and Windows.
Website: github.com/s-andrews/FastQC
Support:
Developer: Simon Andrews
License: GNU General Public License v3.0

FastQC is written in Java. Learn Java with our recommended free books and free tutorials.
Related Software
| Bioinformatics Tools | |
|---|---|
| Bioconductor | Analysis and comprehension of high-throughput genomic data |
| Biopython | Tools for biological computation written in Python |
| BioPerl | Perl tools for computational molecular biology |
| UGENE | Set of integrated bioinformatics software |
| GROMACS | Versatile package to perform molecular dynamics |
| IGV | High-performance visualization genome browser tool |
| BioJava | Provides Java tools for processing biological data |
| InterMine | Integrate biological data sources |
| GATK | Genomic analysis toolkit focused on variant discovery |
| bedtools | Powerful toolset for genome arithmetic |
| EMBOSS | The European Molecular Biology Open Software Suite |
| Clustal Omega | Multiple sequence alignment program |
| BLAST | Algorithm for comparing primary biological sequence information |
| Jalview | Multiple sequence alignment editing, visualisation and analysis |
| Bandage | Visualising de novo assembly graphs |
| GenomeTools | Collection of bioinformatics tools |
| AliView | Alignment viewer and editor |
| mothur | Analyze microbial communities |
| cramino | BAM/CRAM quality evaluation |
| abPOA | Adaptive banded Partial Order Alignment |
| Taverna Workbench | For designing and executing bioinformatics workflows |
| geWorkbench | Software platform for integrated genomic data analysis |
| Bioclipse | Rich-client platform chemistry and biology workbench |
Read our verdict in the software roundup.
Explore our comprehensive directory of recommended free and open source software. Our carefully curated collection spans every major software category.This directory is part of our ongoing series of informative articles for Linux enthusiasts. It features hundreds of detailed reviews, along with open source alternatives to proprietary solutions from major corporations such as Google, Microsoft, Apple, Adobe, IBM, Cisco, Oracle, and Autodesk. You’ll also find interesting projects to try, hardware coverage, free programming books and tutorials, and much more. Discovered a useful open source Linux program that we haven’t covered yet? Let us know by completing this form. |

