Clustal Omega – multiple sequence alignment program

Clustal Omega is a general purpose multiple sequence alignment (MSA) program for protein and DNA/RNA. It produces high quality MSAs and is capable of handling data-sets of hundreds of thousands of sequences in reasonable time.

In default mode, users give a file of sequences to be aligned and these are clustered to produce a guide tree and this is used to guide a “progressive alignment” of the sequences. There are also facilities for aligning existing alignments to each other, aligning a sequence to an alignment and for using a hidden Markov model (HMM) to help guide an alignment of new sequences that are homologous to the sequences used to make the HMM. This latter procedure is referred to as “external profile alignment” or EPA.

Clustal-Omega uses HMMs for the alignment engine, based on the HHalign package from Johannes Soeding. Guide trees are made using an enhanced version of mBed which can cluster very large numbers of sequences in O(N*log(N)) time. Multiple alignment then proceeds by aligning larger and larger alignments using HHalign, following the clustering given by the guide tree.

In its current form Clustal-Omega has been extensively tested for protein sequences, DNA/RNA support has been added since version 1.1.0.

Clustal-Omega accepts 3 types of sequence input: (i) a sequence file with un-aligned or aligned sequences, (ii) profiles (a multiple alignment in a file) of aligned sequences, (iii) a HMM.

Clustal Omega is currently a command line-only tool.

Support: Documentation
Developer: Des Higgins, Fabian Sievers, David Dineen, Andreas Wilm
License: GNU General Public License, version 2

Clustal Omega is written in C. Learn C with our recommended free books and free tutorials.

Return to Bioinformatics Tools

Popular series
Free and Open Source SoftwareThe largest compilation of the best free and open source software in the universe. Each article is supplied with a legendary ratings chart helping you to make informed decisions.
ReviewsHundreds of in-depth reviews offering our unbiased and expert opinion on software. We offer helpful and impartial information.
Alternatives to Proprietary SoftwareReplace proprietary software with open source alternatives: Google, Microsoft, Apple, Adobe, IBM, Autodesk, Oracle, Atlassian, Corel, Cisco, Intuit, and SAS.
GamesAwesome Free Linux Games Tools showcases a series of tools that making gaming on Linux a more pleasurable experience. This is a new series.
Artificial intelligence iconMachine Learning explores practical applications of machine learning and deep learning from a Linux perspective. We've written reviews of more than 40 self-hosted apps. All are free and open source.
Guide to LinuxNew to Linux? Read our Linux for Starters series. We start right at the basics and teach you everything you need to know to get started with Linux.
Alternatives to popular CLI tools showcases essential tools that are modern replacements for core Linux utilities.
System ToolsEssential Linux system tools focuses on small, indispensable utilities, useful for system administrators as well as regular users.
ProductivityLinux utilities to maximise your productivity. Small, indispensable tools, useful for anyone running a Linux machine.
AudioSurveys popular streaming services from a Linux perspective: Amazon Music Unlimited, Myuzi, Spotify, Deezer, Tidal.
Saving Money with LinuxSaving Money with Linux looks at how you can reduce your energy bills running Linux.
Home ComputersHome computers became commonplace in the 1980s. Emulate home computers including the Commodore 64, Amiga, Atari ST, ZX81, Amstrad CPC, and ZX Spectrum.
Now and ThenNow and Then examines how promising open source software fared over the years. It can be a bumpy ride.
Linux at HomeLinux at Home looks at a range of home activities where Linux can play its part, making the most of our time at home, keeping active and engaged.
Linux CandyLinux Candy reveals the lighter side of Linux. Have some fun and escape from the daily drudgery.
DockerGetting Started with Docker helps you master Docker, a set of platform as a service products that delivers software in packages called containers.
Android AppsBest Free Android Apps. We showcase free Android apps that are definitely worth downloading. There's a strict eligibility criteria for inclusion in this series.
Programming BooksThese best free books accelerate your learning of every programming language. Learn a new language today!
Programming TutorialsThese free tutorials offer the perfect tonic to our free programming books series.
Linux Around The WorldLinux Around The World showcases usergroups that are relevant to Linux enthusiasts. Great ways to meet up with fellow enthusiasts.
Stars and StripesStars and Stripes is an occasional series looking at the impact of Linux in the USA.
Notify of

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Inline Feedbacks
View all comments